β catenin d10a8 xp rabbit mab  (Cell Signaling Technology Inc)

Journal: iScience

Article Title: Panx1 deficiency exacerbates GAN diet-induced obesity by destabilizing β-catenin via GSK3β

doi: 10.1016/j.isci.2026.115098

Figure Lengend Snippet: Panx1 increases β-Catenin activity and promotes its nuclear translocation (A) Western blot shows Panx1 overexpression and knockout efficiency (GAPDH as loading control). (B) Glycosylation patterns of exogenous Panx1. (C and D) Total and non-phosphorylated β-catenin levels in whole cell and nuclear fractions (Lamin B1 as loading control). (E) Immunofluorescence of β-catenin nuclear translocation (red; nuclei, blue). Scale bars, 25 μm. (F) qRT-PCR of Wnt/β-catenin downstream gene expression normalized to PPIA. (G) Western blot of Wnt/β-catenin downstream proteins (GAPDH as loading control). Data are mean ± SD, statistics in (C), (D), (F), and (G): ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001, two-sided Student’s t test.

Article Snippet: β-Catenin (D10A8) XP Rabbit mAb , Cell Signaling Technology , Cat# 8480S; RRID: AB_11127855.

Techniques: Activity Assay, Translocation Assay, Western Blot, Over Expression, Knock-Out, Control, Glycoproteomics, Immunofluorescence, Quantitative RT-PCR, Gene Expression

Journal: iScience

Article Title: Panx1 deficiency exacerbates GAN diet-induced obesity by destabilizing β-catenin via GSK3β

doi: 10.1016/j.isci.2026.115098

Figure Lengend Snippet: Panx1 forms complexes with β-Catenin and GSK-3β (A) Western blot of destruction complex proteins in Panx1-OE and Panx1 −/− 3T3-L1 cells (GAPDH as loading control). (B) Co-IP of Panx1 with β-catenin or destruction complex proteins in HEK293T cells expressing Panx1-HA. (C) Co-IP of β-catenin (total or phosphorylated) with GSK-3β in Panx1-OE and Panx1 −/− cells (GAPDH as loading control for input). (D) Western blot showing β-catenin knockdown efficiency in Panx1-OE cells (GAPDH as loading control). (E) Co-IP of Panx1 with GSK-3β after β-catenin knockdown (GAPDH as loading control for input). (F) Predicted 3D structures of Panx1, β-catenin, and GSK-3β complexes showing conformational changes. (G) Ranking scores of binding affinities among Panx1, β-catenin, and GSK-3β. Data are mean ± SD, statistics in (A): ∗ p < 0.05 and ∗∗ p < 0.01, two-sided Student’s t test.

Article Snippet: β-Catenin (D10A8) XP Rabbit mAb , Cell Signaling Technology , Cat# 8480S; RRID: AB_11127855.

Techniques: Western Blot, Control, Co-Immunoprecipitation Assay, Expressing, Knockdown, Binding Assay

Journal: iScience

Article Title: Panx1 deficiency exacerbates GAN diet-induced obesity by destabilizing β-catenin via GSK3β

doi: 10.1016/j.isci.2026.115098

Figure Lengend Snippet: Effect of Panx1 on cell proliferation (A) Cell viability of Panx1-OE and Panx1 −/− cells. (B–D) Flow cytometry analysis of cell cycle distribution (PI staining) with the quantification of G1, S, and G2/M phases. (E) Western blot confirms β-catenin knockdown and effects on downstream proteins in Panx1-OE cells. (F and G) Flow cytometry and quantification of cell cycle distribution after β-catenin knockdown. Each panel represents an independently generated pair of stable lines, and statistical analyses are performed within each matched pair. Data are mean ± SD, statistics in (A), (D), and (G): ∗∗ p < 0.01 and ∗∗∗ p < 0.001, two-sided Student’s t test.

Article Snippet: β-Catenin (D10A8) XP Rabbit mAb , Cell Signaling Technology , Cat# 8480S; RRID: AB_11127855.

Techniques: Flow Cytometry, Staining, Western Blot, Knockdown, Generated